Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.

@article{Xiao2009SimultaneousDO,
  title={Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.},
  author={Xing-long Xiao and Hui Rong Wu and Y Li and Hui-fang Li and Ya-qing He and G. Lily Chen and Jingwei Zhang and Hong Yang and Xiao-Feng Li and Xiaoquan Yang and Yi-gang Yu},
  journal={Journal of virological methods},
  year={2009},
  volume={159 1},
  pages={23-8}
}
Epidemics of acute hemorrhagic conjunctivitis are always explosive and extensive, and have been recognized as a serious international public health problem. Enterovirus 70 and coxsackievirus A24 variant have been identified as the major etiological agents in acute hemorrhagic conjunctivitis outbreaks worldwide. A novel multiplex real-time RT-PCR assay was developed for simultaneous detection, identification and quantitation of enterovirus 70 and coxsackievirus A24 variant. The specificity… CONTINUE READING