Simultaneous detection of X- and Y-bearing human sperm by double fluorescence in situ hybridization.

@article{Han1993SimultaneousDO,
  title={Simultaneous detection of X- and Y-bearing human sperm by double fluorescence in situ hybridization.},
  author={Tong Han and Judith Helen Ford and Graham C. Webb and Sean P. Flaherty and A T Correll and Colin D. Matthews},
  journal={Molecular reproduction and development},
  year={1993},
  volume={34 3},
  pages={308-13}
}
Double fluorescence in situ hybridization (FISH) was used to detect sex chromosomes in decondensed human sperm nuclei. Biotinylated X chromosome specific (TRX) and digoxigenin-labeled Y chromosome specific (HRY) probes were simultaneously hybridized to sperm preparations from 12 normal healthy donors. After the hybridization, the probes were detected immunocytochemically, using two different and independent affinity systems. Ninety-six percent of the 12,636 sperm showed fluorescent labeling, of… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…