Simultaneous detection of S‐adenosylmethionine and S‐adenosylhomocysteine in mouse and rat tissues by capillary electrophoresis

@article{Uthus2003SimultaneousDO,
  title={Simultaneous detection of S‐adenosylmethionine and S‐adenosylhomocysteine in mouse and rat tissues by capillary electrophoresis},
  author={Eric O. Uthus},
  journal={ELECTROPHORESIS},
  year={2003},
  volume={24}
}
  • E. Uthus
  • Published 1 April 2003
  • Chemistry
  • ELECTROPHORESIS
A capillary electrophoresis method for the determination of S‐adenosylmethionine (SAM) and S‐adenosylhomocysteine (SAH) in rat liver and kidney and mouse liver is described. The method can also be used to determine SAM in whole blood. The method provides rapid (approximately 16 min sample to sample) resolution of both compounds in perchloric extracts of tissues. Separation was performed by using an uncoated 50 μm ID capillary with 60 cm total length (50 cm to the detector window). Samples were… 
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19 Citations
Methods Citations
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19 Citations

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Capillary electrophoresis and phenylboronic acid solid phase extraction for the determination of S‐adenosylmethionine/S‐adenosylhomocysteine ratio in human urine
An approach that allows direct analysis of the ratio of S‐adenosylmethionine (SAM) and S‐adenosylhomocysteine (SAH) by using CE is presented. The analytes were extracted on phenylboronic acid phase…
Determination of S‐adenosylmethionine, S‐adenosylhomocysteine, and methylthioadenosine in urine using solvent‐modified micellar electrokinetic chromatography
TLDR
Analysis of 30 urine samples from healthy volunteers showed that the median SAM and SAH levels were 12.1 and 0.73 µM, respectively, and MTA levels, which were determined in urine for the first time, were 0.43 µM; these values correlated well with the SAM level.
Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC–MS/MS and evaluation of their stability in mice tissues
Detection of S‐adenosylhomocysteine and methylation index in blood by capillary electrophoresis
TLDR
After application of meglumine postinjection, this work achieved SAH in‐capillary preconcentration in the HClO4 extracts of erythrocytes, which improved the detection limit of SAH and the methylation index in blood using CE with UV detection.
Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Human Urine by Ion Chromatography with Solid Phase Extraction Based on the Application of Micromolecule Ion-Pairing Agent
Abstract S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) play an important role in various biochemical reactions involving the maintenance of human health and disease prevention. In this…
Evaluation of chemical and diastereoisomeric stability of S-adenosylmethionine in aqueous solution by capillary electrophoresis.
Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometry.
Determination of S-adenosyl-l-methionine in fruits by capillary electrophoresis.
TLDR
Fast measurements of SAM in fruits and juices are favoured by capillary electrophoresis in a 200 : 50 mm glycine-phosphate buffer system, which proved to be very fast and more sensitive compared with the conventional HPLC method.
Analysis of trace degradation products (decarboxylated diastereoisomers) of S‐adenosylmethionine by electrophoresis in capillaries with cationic coatings (N‐methylpolyvinylpyridinium or divalent barium)
TLDR
It was demonstrated that, in the alkylation of 5′‐methylthioadenosine by 3‐bromopropylamine in bromidric acid to dcSAM, another minute impurity was present, proven, via mass spectrometry, to consist of S‐(5′‐adenosyl)‐3‐thiopropylamines (decarboxylated and demethylated (dc‐SAH).
Fast quantification of S-adenosyl-L-methionine in dietary health products utilizing reversed-phase high-performance liquid chromatography: teaching an old method new tricks.
TLDR
The developed protocol is robust, fast, and suitable for the quality control analysis of yeast and commercial S-adenosyl-L-methionine products.
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