Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology.

@article{Kamerbeek1997SimultaneousDA,
  title={Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology.},
  author={J. Kamerbeek and L. Schouls and A. Kolk and M. V. van Agterveld and D. van Soolingen and S. Kuijper and A. Bunschoten and H. Molhuizen and R. Shaw and M. Goyal and J. V. van Embden},
  journal={Journal of clinical microbiology},
  year={1997},
  volume={35 4},
  pages={
          907-14
        }
}
  • J. Kamerbeek, L. Schouls, +8 authors J. V. van Embden
  • Published 1997
  • Biology, Medicine
  • Journal of clinical microbiology
  • Widespread use of DNA restriction fragment length polymorphism (RFLP) to differentiate strains of Mycobacterium tuberculosis to monitor the transmission of tuberculosis has been hampered by the need to culture this slow-growing organism and by the level of technical sophistication needed for RFLP typing. We have developed a simple method which allows simultaneous detection and typing of M. tuberculosis in clinical specimens and reduces the time between suspicion of the disease and typing from 1… CONTINUE READING
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