Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR.

@article{Hassan2006SimultaneousDA,
  title={Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR.},
  author={Mohamed K. Hassan and Arben Myrta and Jaroslav Pol{\'a}k},
  journal={Journal of virological methods},
  year={2006},
  volume={133 2},
  pages={124-9}
}
A pentaplex reverse-transcription polymerase chain reaction (Pentaplex RT-PCR) in a single tube was developed for the simultaneous detection of the pome fruit viruses: Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). This is the first report of the simultaneous detection of all four viruses and host mRNA as an internal specific control. Pentaplex RT-PCR was applied successfully throughout the year, using… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

Similar Papers

Loading similar papers…