Simultaneous detection and differentiation of Staphylococcus species in blood cultures using fluorescence in situ hybridization.

@article{Wang2010SimultaneousDA,
  title={Simultaneous detection and differentiation of Staphylococcus species in blood cultures using fluorescence in situ hybridization.},
  author={Pei Wang},
  journal={Medical principles and practice : international journal of the Kuwait University, Health Science Centre},
  year={2010},
  volume={19 3},
  pages={218-21}
}
OBJECTIVE To develop a new protocol for the simultaneous detection and differentiation of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in blood cultures using fluorescence in situ hybridization (FISH), without cultivation and biotyping. MATERIALS AND METHODS Oligonucleotide probes were used to target the variable regions of the 16S rRNA of S. aureus and CoNS, the probes were labeled with fluorochrome Cy3 (red signal) and fluorescein isothiocyanate (green signal… CONTINUE READING
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