Simultaneous assessment of NF-κB/p65 phosphorylation and nuclear localization using imaging flow cytometry.

@article{Maguire2015SimultaneousAO,
  title={Simultaneous assessment of NF-κB/p65 phosphorylation and nuclear localization using imaging flow cytometry.},
  author={Orla Maguire and Kieran L O'Loughlin and Hans Minderman},
  journal={Journal of immunological methods},
  year={2015},
  volume={423},
  pages={3-11}
}
Aberrant activity of Nuclear Factor-kappaB (NF-κB) is associated with many diseases and is therapeutically targeted. Post-translational modifications, particularly phosphorylation of the RELA/p65 sub-unit, are essential for cytoplasmic to nuclear localization of NF-κB/p65 and initiation of transcription of downstream target genes. Immunoblot and phospho-flow cytometry have been used to study the relationship between phosphorylation motifs and NF-κB activation and microscopic analysis of nuclear… CONTINUE READING

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