Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labels.

@article{Wu2014SimultaneousAF,
  title={Simultaneous aptasensor for multiplex pathogenic bacteria detection based on multicolor upconversion nanoparticles labels.},
  author={Shijia Wu and Nuo Duan and Zhao Hui Shi and Congcong Fang and Zhouping Wang},
  journal={Analytical chemistry},
  year={2014},
  volume={86 6},
  pages={3100-7}
}
A highly sensitive and specific multiplex method for the simultaneous detection of three pathogenic bacteria was fabricated using multicolor upconversion nanoparticles (UCNPs) as luminescence labels coupled with aptamers as the molecular recognition elements. Multicolor UCNPs were synthesized via doping with various rare-earth ions to obtain well-separated emission peaks. The aptamer sequences were selected using the systematic evolution of ligands by exponential enrichment (SELEX) strategy for… CONTINUE READING

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