Simultaneous and selective detection of two major soft rot pathogens of potato: Pectobacterium atrosepticum (Erwinia carotovora subsp. atrosepticum) and Dickeya spp. (Erwinia chrysanthemi)

@article{Diallo2009SimultaneousAS,
  title={Simultaneous and selective detection of two major soft rot pathogens of potato: Pectobacterium atrosepticum (Erwinia carotovora subsp. atrosepticum) and Dickeya spp. (Erwinia chrysanthemi)},
  author={St{\'e}phanie Diallo and Xavier Latour and Anne Groboillot and Bruno Smadja and Patricia Copin and Nicole Orange and Marc G J Feuilloley and Sylvie Chevalier},
  journal={European Journal of Plant Pathology},
  year={2009},
  volume={125},
  pages={349-354}
}
Dickeya spp. and Pectobacterium atrosepticum are major pathogens of potato. Current methods to detect these soft-rotting bacteria require separate identification steps. Here we describe a simple method allowing simultaneous detection of both pathogens based on multiplex PCR. The sensitivity of the primer sets was first examined on purified genomic DNA of the type strains Dickeya chrysanthemi 2048T and P. atrosepticum 1526T. The specificity and detection limits of the primer sets were… CONTINUE READING

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