Simultaneous and rapid analysis of bile acids including conjugates by supercritical fluid chromatography coupled to tandem mass spectrometry.

@article{Taguchi2013SimultaneousAR,
  title={Simultaneous and rapid analysis of bile acids including conjugates by supercritical fluid chromatography coupled to tandem mass spectrometry.},
  author={Kaori Taguchi and Eiichiro Fukusaki and Takeshi Bamba},
  journal={Journal of chromatography. A},
  year={2013},
  volume={1299},
  pages={103-9}
}
A number of analysis methods for bile acids using LC-MS and GC-MS have been reported. However, there is no reported method for the simultaneous analysis of bile acids using supercritical fluid chromatography (SFC). In this study, we have successfully developed a rapid method using SFC coupled to electrospray ionization tandem mass spectrometry (ESI-MS/MS) for comprehensive bile acid profiling. 25 bile acids including glycine and taurine conjugates were analyzed simultaneously within 13min. The… CONTINUE READING
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