Simultaneous and label-free determination of wild-type and mutant p53 at a single surface plasmon resonance chip preimmobilized with consensus DNA and monoclonal antibody.

@article{Wang2009SimultaneousAL,
  title={Simultaneous and label-free determination of wild-type and mutant p53 at a single surface plasmon resonance chip preimmobilized with consensus DNA and monoclonal antibody.},
  author={Yongcan Wang and Xu Chao Zhu and Minghua Wu and Ning Xia and Jianxiu Wang and Feimeng Zhou},
  journal={Analytical chemistry},
  year={2009},
  volume={81 20},
  pages={8441-6}
}
Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-stranded (ds-) DNA and a monoclonal antibody. The high affinity of the consensus ds-DNA to the wild-type p53 and the antibody to total p53 results in remarkably low detection levels (10.6 and 1.06 pM… CONTINUE READING