Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses.

@article{Olmos2002SimultaneousAC,
  title={Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses.},
  author={Antonio Olmos and Edson Bertolini and Mariano Cambra},
  journal={Journal of virological methods},
  year={2002},
  volume={106 1},
  pages={51-9}
}
A new and highly sensitive method for the amplification of viral RNA targets from plant material has been developed and patented. This technique called Co-operational amplification (Co-PCR) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. The reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination… CONTINUE READING

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