Simultaneous analysis of the main markers of nitrogen status in dairy cow's urine using hydrophilic interaction chromatography and tandem mass spectrometry detection.

Abstract

Microbial protein synthesis and nitrogen balance status in ruminants can be evaluated by the presence of metabolites in urine. This work aims to develop and validate a simple and sensitive method for simultaneous determination of nine markers of nitrogen status in ruminant's urine using hydrophilic interaction liquid chromatography coupled with electrospray… (More)
DOI: 10.1016/j.chroma.2012.07.094

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