Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography.

@article{Takegawa2011SimultaneousAO,
  title={Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography.},
  author={Yasuhiro Takegawa and Kayo Araki and Naoki Fujitani and Jun-ichi Furukawa and Hiroaki Sugiyama and Hideaki Sakai and Yasuro Shinohara},
  journal={Analytical chemistry},
  year={2011},
  volume={83 24},
  pages={9443-9}
}
Glycosaminoglycans (GAGs) play important roles in cell adhesion and growth, maintenance of extracellular matrix (ECM) integrity, and signal transduction. To fully understand the biological functions of GAGs, there is a growing need for sensitive, rapid, and quantitative analysis of GAGs. The present work describes a novel analytical technique that enables high throughput cellular/tissue glycosaminoglycomics for all three families of uronic acid-containing GAGs, hyaluronan (HA), chondroitin… CONTINUE READING