Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny

@article{Lassner1989SimultaneousAO,
  title={Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny},
  author={Michael W Lassner and Peter Peterson and John I Yoder},
  journal={Plant Molecular Biology Reporter},
  year={1989},
  volume={7},
  pages={116-128}
}
We describe the simultaneous amplification of different segments of foreign DNA in transgenic plants using the polymerase chain reaction (PCR). We used PCR to simultaneously amplify different regions of transformed T-DNA in order to assay the integrity of transformed constructions in primary tomato transformants. We also used simultaneous PCR amplification to examine the segregation of transformed sequences in progeny of primary transformants. A tomato transformant containing the maize… CONTINUE READING

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Polymerase chain reaction with single-sided specificity: analysis of T cell receptor d chain

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