Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4'-dimethylaminophenylazo)benzoic acid as a dark quencher dye.

@article{Kreuzer2001SimultaneousAQ,
  title={Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4'-dimethylaminophenylazo)benzoic acid as a dark quencher dye.},
  author={K A Kreuzer and Andreas Bohn and Joachim Lupberger and J{\'e}r{\^o}me Solassol and Philipp le Coutre and Christian A. Schmidt},
  journal={Clinical chemistry},
  year={2001},
  volume={47 3},
  pages={486-90}
}
BACKGROUND Despite the many advantages of real-time fluorescence reverse transcription-PCR (RT-PCR) as a quantitative analytical tool, simultaneous quantification of target and reference templates within one reaction has not been reported. We developed such an assay with an internal reference template. METHODS For quantification of target and reference sequences, we used two fluorescent probes in one reaction vessel on an ABI PRISM 7700 SDS instrument. Fluorescent probes were labeled with… CONTINUE READING