Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells

  title={Simultaneous Transcriptional Profiling of Bacteria and Their Host Cells},
  author={Michael S. Humphrys and Todd Creasy and Yezhou Sun and Amol Carl Shetty and Marcus C. Chibucos and Elliott Franco Dr{\'a}bek and Claire M. Fraser and Umar Bin Farooq and Naomi Sengamalay and Sandy Ott and Huizhong Shou and Patrik M. Bavoil and Anup Mahurkar and Garry S. A. Myers},
  booktitle={PloS one},
We developed an RNA-Seq-based method to simultaneously capture prokaryotic and eukaryotic expression profiles of cells infected with intracellular bacteria. As proof of principle, this method was applied to Chlamydia trachomatis-infected epithelial cell monolayers in vitro, successfully obtaining transcriptomes of both C. trachomatis and the host cells at 1 and 24 hours post-infection. Chlamydiae are obligate intracellular bacterial pathogens that cause a range of mammalian diseases. In humans… CONTINUE READING
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