Simultaneous RT/PCR detection and differentiation of arabis mosaic and grapevine fanleaf nepoviruses in grapevines with a single pair of primers.

@article{Wetzel2002SimultaneousRD,
  title={Simultaneous RT/PCR detection and differentiation of arabis mosaic and grapevine fanleaf nepoviruses in grapevines with a single pair of primers.},
  author={T Wetzel and Rahma Jardak and Laurent Meunier and Abdelwahed Ghorbel and Goetz M. Reustle and Gabriele Krczal},
  journal={Journal of virological methods},
  year={2002},
  volume={101 1-2},
  pages={
          63-9
        }
}
The movement protein genes from several isolates of ArMV and GFLV of different geographical origins were amplified by RT/PCR using degenerate primers, cloned and sequenced. A single pair of degenerate primers was designed from these sequences to allow the simultaneous amplification of parts of the movement protein genes of ArMV and GFLV. Their use in an immunocapture-RT/PCR for the detection of ArMV or GFLV in infected grapevines proved to be ten times more sensitive than the corresponding ArMV… CONTINUE READING
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