Simultaneous Quantification of Anandamide and Other Endocannabinoids in Dorsal Vagal Complex of Rat Brainstem by LC-MS.

@article{Chen2009SimultaneousQO,
  title={Simultaneous Quantification of Anandamide and Other Endocannabinoids in Dorsal Vagal Complex of Rat Brainstem by LC-MS.},
  author={Jing Chen and Kalpana S. Paudel and Andrei V. Derbenev and Bret N Smith and Audra L Stinchcomb},
  journal={Chromatographia},
  year={2009},
  volume={69 1-2},
  pages={1-7}
}
A quantitative method has been developed and validated for the simultaneous determination of anandamide (AEA), docosatetraenylethanolamide (DEA) and N-arachidonyldopamine (NADA) in dorsal vagal complex (DVC) of rat brainstem by liquid chromatographic-electrospray ionization mass spectrometry. The analytes were extracted from the tissue samples of rat brainstem by a single step liquid extraction technique using acetonitrile. The chromatographic separation was conducted on a C18 column using a… CONTINUE READING