Simultaneous Qualitative And Quantitative Identification Of Multiple Targets By Polymerase Chain Reaction On Microarray

@inproceedings{Zakharova2008SimultaneousQA,
  title={Simultaneous Qualitative And Quantitative Identification Of Multiple Targets By Polymerase Chain Reaction On Microarray},
  author={Natalia V. Zakharova and Alexei L. Drobyshev and Dmitry A Khodakov and Anna I. Myznikova and Irina A Gorban},
  year={2008}
}
An approach for multiplex qualitative and quantitative microarray-based PCR analysis has been proposed. The characteristics of PCR executed on a gel-based oligonucleotide microarray with immobilized forward primers and a single common reverse primer in solution were investigated for several DNA targets. One-stage multiplex on-chip PCR was studied for simultaneous amplification of herpes simplex viruses types 1 and 2, cytomegalovirus DNA, and bacteriophage lambda DNA as an internal control… CONTINUE READING