Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy.

Abstract

Apart from their essential role in generating ATP, mitochondria also act as local calcium (Ca2+) buffers to tightly regulate intracellular Ca2+ concentration. To do this, mitochondria utilize the electrochemical potential across their inner membrane (ΔΨm) to sequester Ca2+. The influx of Ca2+ into the mitochondria stimulates three rate-limiting… (More)
DOI: 10.3791/55166

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Cite this paper

@article{McKenzie2017SimultaneousMO, title={Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy.}, author={Matthew McKenzie and Sze Chieh Lim and Michael R Duchen}, journal={Journal of visualized experiments : JoVE}, year={2017}, volume={119} }