Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS.

@article{SimesPires2017SimultaneousMO,
  title={Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS.},
  author={Claudia Avello Sim{\~o}es-Pires and Vincent Zwick and Sylvian Cretton and Muriel Cuendet},
  journal={Journal of visualized experiments : JoVE},
  year={2017},
  volume={126}
}
The search for new histone deacetylase (HDAC) inhibitors is of increasing interest in drug discovery. Isoform selectivity has been in the spotlight since the approval of romidepsin, a class I HDAC inhibitor for cancer therapy, and the clinical investigation of HDAC6-specific inhibitors for multiple myeloma. The present method is used to determine the inhibitory activity of test compounds on HDAC1 and HDAC6 in cells. The isoform activity is measured using the ultra-high-performance liquid… CONTINUE READING
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