Simultaneous Isolation of Glial and Neuronal Fractions from Rat Brain Homogenates: Comparison of High-Affinity L-Glutamate Transport Properties

Abstract

An improved three-step Percoll density gradient centrifugation technique is described for simultaneous isolation of glial plasmalemmal vesicles (GPV) and synaptosomal vesicles (SYN) from a rat brain homogenate. While electron microscopy revealed that fractions contained intact vesicles with markedly distinct morphological features, measures of high-affinity… (More)
DOI: 10.1023/A:1022413823183

Topics

6 Figures and Tables

Cite this paper

@article{Daniels1998SimultaneousIO, title={Simultaneous Isolation of Glial and Neuronal Fractions from Rat Brain Homogenates: Comparison of High-Affinity L-Glutamate Transport Properties}, author={Kellye K. Daniels and Thomas W. Vickroy}, journal={Neurochemical Research}, year={1998}, volume={23}, pages={103-113} }