Simultaneous Identification of DNA and RNA Viruses Present in Pig Faeces Using Process-Controlled Deep Sequencing

@inproceedings{Sachsenrder2012SimultaneousIO,
  title={Simultaneous Identification of DNA and RNA Viruses Present in Pig Faeces Using Process-Controlled Deep Sequencing},
  author={Jana Sachsenr{\"o}der and Sven O Twardziok and Jens A Hammerl and Pawel Janczyk and Paul Wrede and Stefan Hertwig and R Johne},
  booktitle={PloS one},
  year={2012}
}
BACKGROUND Animal faeces comprise a community of many different microorganisms including bacteria and viruses. Only scarce information is available about the diversity of viruses present in the faeces of pigs. Here we describe a protocol, which was optimized for the purification of the total fraction of viral particles from pig faeces. The genomes of the purified DNA and RNA viruses were simultaneously amplified by PCR and subjected to deep sequencing followed by bioinformatic analyses. The… CONTINUE READING

11 Figures & Tables

Connections & Topics

Mentioned Connections BETA
They mainly originated from bacteriophages ( 73.9% ) and mammalian viruses ( 23.9% ) ; 0.8% of the sequences showed identities to plant viruses .
They mainly originated from bacteriophages ( 73.9% ) and mammalian viruses ( 23.9% ) ; 0.8% of the sequences showed identities to plant viruses .
The most abundant detected porcine viruses were kobuvirus , rotavirus C , astrovirus , enterovirus B , sapovirus and picobirnavirus .
The most abundant detected porcine viruses were kobuvirus , rotavirus C , astrovirus , enterovirus B , sapovirus and picobirnavirus .
The most abundant detected porcine viruses were kobuvirus , rotavirus C , astrovirus , enterovirus B , sapovirus and picobirnavirus .
The most abundant detected porcine viruses were kobuvirus , rotavirus C , astrovirus , enterovirus B , sapovirus and picobirnavirus .
All Topics