Simultaneous Determination of Cortisol and Cortisone from Human Serum by Liquid Chromatography-Tandem Mass Spectrometry

@inproceedings{Lee2014SimultaneousDO,
  title={Simultaneous Determination of Cortisol and Cortisone from Human Serum by Liquid Chromatography-Tandem Mass Spectrometry},
  author={Sanghoo Lee and Hwan-Sub Lim and Hye-Jin Shin and Seol-A Kim and Jimyeong Park and Hyun-Chul Kim and Hyogyeong Kim and Hyung joo Kim and Yun-Tae Kim and Kyoung-Ryul Lee and Young-Jin Kim},
  booktitle={Journal of analytical methods in chemistry},
  year={2014}
}
A fast, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was validated and then the levels of cortisol and cortisone from sera of healthy adults were determined by the LC-MS/MS method. One hundred μ L of serum sample was directly extracted by adding 2 mL ethyl acetate, followed by chromatographic separation on a C18 column with a mobile phase consisting of 5 mM ammonium acetate and methanol (25 : 75, v/v). The precision, accuracy, and average recovery of… CONTINUE READING

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