Simultaneous Determination of Carbamazepine, Phenobarbital and Their Major Metabolites in Serum, Brain Tissue and Urine by High-Performane Liquid Chromatographya

@inproceedings{MendezAlvarez1987SimultaneousDO,
  title={Simultaneous Determination of Carbamazepine, Phenobarbital and Their Major Metabolites in Serum, Brain Tissue and Urine by High-Performane Liquid Chromatographya},
  author={E Mendez-Alvarez and Ramon Soto-otera and German Sierra-marcuňo},
  year={1987}
}
Abstract A high-performance liquid chromatographic method is described for the simultaneous measurement of carbamazepine, phenobarbital and their major metabolites in small samples of serum, brain tissue and urine. This involves solvent extraction, reversed-phase chromatography and ultraviolet detection at 195 nm. Glucuronides in urine are hydrolyzed by enzymatic cleavage with β-glucuranidase. Quantitation is based on the peak-height ratio of the analyte to its internal standard (10, 11… CONTINUE READING

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