Simultaneous Detection of Tricyclic Antidepressants using Higher Performance Liquid Chromatography


Despite the introduction of newer antidepressants, tricyclic antidepressants are still widely used for the treatment of major depression, chronic pain and anxiety. And is the most leading cause of drug-induced death in many patients. Monitoring their blood/plasma concentration is therefore a priority in forensic toxicology. In this study, a quick and rapid method for simultaneous detection and quantification of three TCAs (desipramine, nortriptyline and amitriptyline) in whole blood was developed and validated on HPLC-UV under a gradient elution, using a C18 column and a mobile phase mixture of ACN (35% v/v) and a pH 3.5, 50mM phosphate buffer (65% v/v). The evaluated validation parameters were satisfactorily accepted in accordance with ICH and FDA guidelines. The determination coefficients were more than 0.99 within the tested concentration range (0.1–1mgL -1 ), LOD and LOQ were between 0.0027-0.0051 and 0.27-0.051 respectively, Accuracy expressed as % Er was found to be between 0.1-7% and precision expressed as %CoV was found to be between 2-10%. All compounds were satisfactorily determined in one single injection within 20 min. sample pre-treatment was optimised on three sets of cartridges (C2,C18 and Strata-X) on a solid phase extractions, with C18 giving the best recovery for all analytes ranging from 60-105%. The developed method proved to be suitable for routine work and it was used to successfully analyse forensic blood samples.

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Cite this paper

@inproceedings{Sulayman2017SimultaneousDO, title={Simultaneous Detection of Tricyclic Antidepressants using Higher Performance Liquid Chromatography}, author={Jamiu Mustapha Sulayman and Bashir Abdulkadir}, year={2017} }