Simultaneous Detection of Salmonella , Listeria monocytogenes , and Staphylococcus aureus by Multiplex Real-Time PCR Assays Using High-Resolution Melting

@inproceedings{Xiao2014SimultaneousDO,
  title={Simultaneous Detection of Salmonella , Listeria monocytogenes , and Staphylococcus aureus by Multiplex Real-Time PCR Assays Using High-Resolution Melting},
  author={Xing-long Xiao and Li Zhang and Hui Wu and Yi-gang Yu and Yu-qian Tang and Dong-mei Liu and Xiao-Feng Li},
  year={2014}
}
A method combining multiplex real-time polymerase chain reaction (PCR) with high-resolution melting (HRM) analysis for rapid and specific simultaneous detection of Salmonella, Listeria monocytogenes, and Staphylococcus aureus was developed. The method included a meltingcurve analysis of products and was evaluated by specificity, sensitivity and reproducibility analyses. Sensitivity and reproducibility analyses was both conducted by genomic DNA extracted from serial dilutions for each target… CONTINUE READING