Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells

  title={Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells},
  author={Paul H. Tewson and Mara Westenberg and Yongxin Zhao and Robert E. Campbell and Anne Marie Quinn and Thomas E Hughes},
  booktitle={PloS one},
Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca(2+) dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca… CONTINUE READING
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