Simultaneous Amplification and Detection of Specific DNA Sequences

  title={Simultaneous Amplification and Detection of Specific DNA Sequences},
  author={Russell Higuchi and Gavin Dollinger and P. Sean Walsh and R C Griffith},
We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences can be detected without opening the reaction tube. This enhancement requires the addition of ethidium bromide (EtBr) to a PCR. Since the fluorescence of EtBr increases in the presence of double–stranded (ds) DNA an increase in fluorescence in such a PCR indicates a positive amplification, which can be easily monitored externally. In fact, amplification can be continuously monitored in order to follow its… Expand
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    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1989
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