Simultaneous Absolute Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases as a Novel Approach for the Characterization of Individual Human Liver: Comparison with mRNA Levels and Activities

  title={Simultaneous Absolute Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases as a Novel Approach for the Characterization of Individual Human Liver: Comparison with mRNA Levels and Activities},
  author={Sumio Ohtsuki and Olaf Schaefer and Hirotaka Kawakami and Tae Inoue and Stephanie Liehner and Asami Saito and Naoki Ishiguro and Wataru Kishimoto and Eva Ludwig-Schwellinger and Thomas Ebner and Tetsuya Terasaki},
  journal={Drug Metabolism and Disposition},
  pages={83 - 92}
The purpose of the present study was to determine the absolute protein expression levels of multiple drug-metabolizing enzymes and transporters in 17 human liver biopsies, and to compare them with the mRNA expression levels and functional activities to evaluate the suitability of the three measures as parameters of hepatic metabolism. Absolute protein expression levels of 13 cytochrome P450 (P450) enzymes, NADPH-P450 reductase (P450R) and 6 UDP-glucuronosyltransferase (UGT) enzymes in… 

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  • 2005