Simultaneous “One Pot” Expressed Protein Ligation and CuI‐Catalyzed Azide/Alkyne Cycloaddition for Protein Immobilization

@article{Steinhagen2011SimultaneousP,
  title={Simultaneous “One Pot” Expressed Protein Ligation and CuI‐Catalyzed Azide/Alkyne Cycloaddition for Protein Immobilization},
  author={Max Steinhagen and Kai Holland‐Nell and Morten Meldal and Annette G. Beck‐Sickinger},
  journal={ChemBioChem},
  year={2011},
  volume={12}
}
Proteins, enzymes in particular, are becoming more and more important in synthesis and the creation of stereoand regioselectively novel products with high specificity. They also play an important role in the development of diagnostic systems, microarrays and biosensors. The anchoring of proteins to solid supports can facilitate these systems, but to maintain the native properties and homogeneity, site specific immobilization is preferred, which in turn calls for site specific introduction of… 

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