Simulation and economic assessment of large-scale enzymatic N-acetyllactosamine manufacture

  title={Simulation and economic assessment of large-scale enzymatic N-acetyllactosamine manufacture},
  author={Masih Karimi Alavijeh and A.S. Meyer and S{\'i}lvia Gras and Sandra E. Kentish},
  journal={Biochemical Engineering Journal},
N-acetyllactosamine (LacNAc) is an important lactose-derived molecule which can act as an effective prebiotic. In this study a process for the enzymatic synthesis and downstream purification of LacNAc was designed based on the use of thermostable β-galactosidases from Bacillus circulans (BgaD-D), Thermus thermophilus HB27 or Pyrococcus furiosus (CelB) respectively. Four configurations for the purification stage were simulated; anion-exchange chromatography, an activated charcoal-Celite column… Expand
3 Citations
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