Simulated acidosis does not impair 1,25-dihydroxyvitamin D3 production by cultured kidney cells

@article{Cunningham1987SimulatedAD,
  title={Simulated acidosis does not impair 1,25-dihydroxyvitamin D3 production by cultured kidney cells},
  author={Dr. J. Cunningham and Graeme Griffin and Louis V. Avioli},
  journal={Calcified Tissue International},
  year={1987},
  volume={41},
  pages={342-345}
}
Cultured mouse kidney cells grown in serum-free medium were used to assess the metabolism of 25-hydroxyvitamin D3 in the presence of simulated metabolic acidosis. Kidney epithelial cells isolated from 4–6 week old mice were grown to confluence in a defined serum-free medium at pH 7.4. The confluent monolayers were incubated with tritiated 25-hydroxyvitamin D3 for 6 hours, the samples were extracted, and vitamin D metabolites were separated and quantitated by high pressure liquid chromatography… CONTINUE READING