Simplified sample processing combined with a sensitive one-tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens.

@article{Mathis1997SimplifiedSP,
  title={Simplified sample processing combined with a sensitive one-tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens.},
  author={A. Gschaedler Mathis and Rainer K. Weber and Heinz Kuster and Rudolf Speich},
  journal={Journal of clinical microbiology},
  year={1997},
  volume={35 7},
  pages={1691-5}
}
Early diagnosis of Pneumocystis carinii pneumonia, a life-threatening complication in immunosuppressed patients, may lower morbidity and mortality. We have developed a one-tube nested PCR assay for the detection of P. carinii in respiratory specimens. Four primers were selected from the sequence of the small-subunit rRNA gene of P. carinii to amplify a 265-bp fragment, and their specificities for P. carinii were confirmed by both theoretical evaluations (by computer-assisted comparison with the… CONTINUE READING
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