Simplified procedures for detection of amplified DNA using fluorescent label incorporation and reverse probing.

@article{Woolford1992SimplifiedPF,
  title={Simplified procedures for detection of amplified DNA using fluorescent label incorporation and reverse probing.},
  author={Alison Woolford and Jeremy W. Dale},
  journal={FEMS microbiology letters},
  year={1992},
  volume={78 2-3},
  pages={311-6}
}
Conventional methods of detecting polymerase chain reaction (PCR) products require equipment and expertise which may not be available in diagnostic bacteriology laboratories, especially in developing countries. To this end we have examined other methods of product detection, including fluorescein-12-dUTP incorporation during PCR amplification, and reverse probing, where the PCR product is used as the probe in a scaled down hybridization with a fixed capture probe consisting of a fragment… CONTINUE READING

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