Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.

@article{Suchland1991SimplifiedMC,
  title={Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.},
  author={Robert J Suchland and Walter E. Stamm},
  journal={Journal of clinical microbiology},
  year={1991},
  volume={29 7},
  pages={1333-8}
}
Serotyping of Chlamydia trachomatis strains usually requires three to six serial passages in shell vials to attain sufficient antigen for typing procedures. To circumvent this problem, we developed a rapid low-passage method for serotyping of C. trachomatis clinical isolates. Isolates with an inclusion count of greater than or equal to 500 per well in primary isolation were inoculated directly onto cell culture monolayers in microtiter plates for typing. Primary isolates with a lower initial… CONTINUE READING

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