Simplified method for dissolved DNA determination in aquatic environments.

  title={Simplified method for dissolved DNA determination in aquatic environments.},
  author={Mary F. Deflaun and J H Paul and D Davis},
  journal={Applied and environmental microbiology},
  volume={52 4},
A method was developed for the determination of dissolved DNA in aquatic environments. The method is based upon the concentration of dissolved DNA by ethanol precipitation of 0.2-mum-pore-size filtered water. The DNA in concentrated extracts was quantified by the fluorescence of Hoechst 33258-DNA complexes. Fluorescence not attributable to DNA was corrected for by DNase I digestion of the extracts and averaged 25% of the total fluorescence for all samples. The effectiveness of the procedure for… CONTINUE READING

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