Simplified high-performance liquid chromatography-mass spectrometry assay for measurement of tacrolimus and its metabolites and cross-validation with microparticle enzyme immunoassay.

@article{Gonschior1995SimplifiedHL,
  title={Simplified high-performance liquid chromatography-mass spectrometry assay for measurement of tacrolimus and its metabolites and cross-validation with microparticle enzyme immunoassay.},
  author={A. K. Gonschior and Uwe Christians and Michael Bjorn Winkler and Hans Martin Schiebel and Alfred Linck and K. F. Sewing},
  journal={Therapeutic drug monitoring},
  year={1995},
  volume={17 5},
  pages={504-10}
}
In this study, a modified, specific assay for measurement of tacrolimus and its metabolites in blood and urine from transplant patients using high-performance liquid chromatography (HPLC) linked to mass spectrometry (MS) is described. Samples were prepared for HPLC-MS by modified solid-liquid extraction. The original two-step washing procedure was replaced… CONTINUE READING