Simplified capillary isoelectric focusing with chemical mobilization for intact protein analysis.

@article{Zhu2017SimplifiedCI,
  title={Simplified capillary isoelectric focusing with chemical mobilization for intact protein analysis.},
  author={Guijie Zhu and Liangliang Sun and Norman J. Dovichi},
  journal={Journal of separation science},
  year={2017},
  volume={40 4},
  pages={
          948-953
        }
}
We report a capillary isoelectric focusing system based on a sequential injection method for simplified chemical mobilization. This system was coupled to an ion trap mass spectrometer with an electrokinetically pumped nanoelectrospray interface. The nanoelectrospray emitter employed an acidic sheath electrolyte. To simplify focusing and mobilization, a plug… CONTINUE READING