Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling.

  title={Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling.},
  author={Sara Najmeh and Jonathan J. Cools-Lartigue and Betty Giannias and Jonathan D. Spicer and Lorenzo Edwin Ferri},
  journal={Journal of visualized experiments : JoVE},
Neutrophil Extracellular Traps (NETs) have been recently identified as part of the neutrophil's antimicrobial armamentarium. Apart from their role in fighting infections, recent research has demonstrated that they may be involved in many other disease processes, including cancer progression. Isolating purified NETs is a crucial element to allow the study of these functions. In this video, we demonstrate a simplified method of cell free NET isolation from human whole blood using readily… CONTINUE READING