Simplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation

@inproceedings{Kim2006SimplifiedEG,
  title={Simplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation},
  author={Seok Hyun Kim and Seung Yup Ku and Ki Cheong Sung and Moon Joo Kang and Sung Ah Kim and Hee Sun Kim and Sun Kyung Oh and Byung Chul Jee and Chang Suk Suh and Young Min Choi and Jung Gu Kim and Shin Yong Moon},
  booktitle={Yonsei medical journal},
  year={2006}
}
This study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compared with those of the slow freezing method using 1.5M DMSO. Then, conventional EM grid vitrification… CONTINUE READING
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