Simple reversed-phase high-performance liquid chromatography quantitation of ganciclovir in human serum and urine.

@article{Page1996SimpleRH,
  title={Simple reversed-phase high-performance liquid chromatography quantitation of ganciclovir in human serum and urine.},
  author={Theodore Page and Chris Sherwood and John Dennis Connor and T L Tarnowski},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1996},
  volume={675 2},
  pages={342-6}
}
A fast, simple, and cost-effective HPLC method for the quantitation of the antiviral drug ganciclovir is described. The serum samples are extracted with perchloric acid and neutralized with potassium phosphate buffer, and urine samples are diluted with distilled water. A reversed-phase column with isocratic elution by 15 mM potassium phosphate buffer (pH 2.5) containing 0.25% acetonitrile is used to separate ganciclovir; quantitation is by UV absorbance at 254 nm. Total turnaround time is 22… CONTINUE READING