Simple radiometric techniques for rapid detection of herpes simplex virus type 1 in WI-38 cell culture.

@article{DAntonio1976SimpleRT,
  title={Simple radiometric techniques for rapid detection of herpes simplex virus type 1 in WI-38 cell culture.},
  author={N L D'Antonio and Min-fu Tsan and Patricia Charache and Steven M. Larson and Henry N. Wagner},
  journal={Journal of nuclear medicine : official publication, Society of Nuclear Medicine},
  year={1976},
  volume={17 6},
  pages={503-7}
}
Two radiometric techniques were developed for detecting the presence of herpes simplex virus type 1 in stationary monolayers of the diploid cell line WI-38. The time of detection was compared to that obtained from visual examination for cytopathic effects in the same cell line. Oxidation of 14C-1-glucose in infected and uninfected cells was determined by 14CO2 production, measured by an ionization chamber, and DNA synthesis was determined by 3H-thymidine incorporation, measured by scintillation… CONTINUE READING
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