Simple protocol for extracting nuclear DNA from single embryos of a marine snail.

@article{Simpson1999SimplePF,
  title={Simple protocol for extracting nuclear DNA from single embryos of a marine snail.},
  author={R. Jamie Simpson and Craig Stephen Wilding and John W Grahame},
  journal={BioTechniques},
  year={1999},
  volume={26 6},
  pages={1050-2}
}
1050 BioTechniques Vol. 26, No. 6 (1999) DNA in solution were used instead of 10.75 μL of ddH2O and 8.5 μL of DNA in solution. The re-amplified eluted band was electrophoresed, gel-purified and cloned into a pGEM-T vector (Promega, Madison, WI, USA). We then identified clones containing recombinant plasmids, isolated plasmid DNA and used the insert of an individual clone as a hybridization probe in northern blot analysis. A single hybridizing mRNA of approximately 520 bases was identified in… CONTINUE READING

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We then identified clones containing recombinant plasmids , isolated plasmid DNA and used the insert of an individual clone as a hybridization probe in northern blot analysis .
We then identified clones containing recombinant plasmids , isolated plasmid DNA and used the insert of an individual clone as a hybridization probe in northern blot analysis .
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