Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines.

@article{Iiizumi2006SimpleOM,
  title={Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines.},
  author={Susumu Iiizumi and Yuji Nomura and Sairei So and Koichi Uegaki and Kayoko Aoki and Kei-ichi Shibahara and Noritaka Adachi and Hideki Koyama},
  journal={BioTechniques},
  year={2006},
  volume={41 3},
  pages={
          311-6
        }
}
Targeted gene disruption is a powerful tool for studying gene function in cells and animals. In addition, this technology includes a potential to correct disease-causing mutations. However, constructing targeting vectors is a laborious step in the gene-targeting strategy, even apart from the low efficiency of homologous recombination in mammals. Here, we introduce a quick and simplified method to construct targeting vectors. This method is based on the commercially available MultiSite Gateway… CONTINUE READING