Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.

@article{Uesaka1994SimpleMO,
  title={Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose.},
  author={Yoshihiko Uesaka and Yasuo Otsuka and Zhi-Hong Lin and Satoshi Yamasaki and Junichi Yamaoka and Hisao Kurazono and Yoshifumi Takeda},
  journal={Microbial pathogenesis},
  year={1994},
  volume={16 1},
  pages={71-6}
}
A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis.