Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription.

@article{Ichihashi2015SimpleIO,
  title={Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription.},
  author={Norikazu Ichihashi and Shungo Kobori and Tetsuya Yomo},
  journal={Methods in enzymology},
  year={2015},
  volume={550},
  pages={93-107}
}
Aptazymes are artificially synthesized ribozymes that catalyze reactions in response to ligand binding. Certain types of aptazymes can be utilized as RNA-based regulators of gene expression. These aptazymes contain a sequestered ribosome-binding site (rbs) and release the rbs through self-cleavage in response to ligand binding, inducing the expression of the downstream gene. One of the most important properties of aptazymes as gene expression regulators is their signal-to-noise ratio (S/N ratio… CONTINUE READING