Simple high-performance liquid chromatography method for the simultaneous determination of serum caffeine and paraxanthine following rapid sample preparation.

@article{Holland1998SimpleHL,
  title={Simple high-performance liquid chromatography method for the simultaneous determination of serum caffeine and paraxanthine following rapid sample preparation.},
  author={Diane T Holland and K A Godfredsen and Theodore Page and John Dennis Connor},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={707 1-2},
  pages={105-10}
}
A simple reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous determination of caffeine and paraxanthine in human serum is described. Serum proteins are precipitated with perchloric acid and the resulting supernatant neutralized for direct injection onto an HPLC column. The method uses a phosphate-methanol mobile phase… CONTINUE READING