Simple high-performance liquid chromatographic method to analyze serum creatinine has several advantages over the Jaffé picric acid reaction as demonstrated with a cimetidine dose response in rhesus monkeys.

@article{Johns2001SimpleHL,
  title={Simple high-performance liquid chromatographic method to analyze serum creatinine has several advantages over the Jaff{\'e} picric acid reaction as demonstrated with a cimetidine dose response in rhesus monkeys.},
  author={B. A. Johns and Theodore P. Broten and Maria T. Stranieri and Marie A Holahan and Jacquelynn J. Cook},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={2001},
  volume={759 2},
  pages={
          343-8
        }
}
A simple method for creatinine determination was developed using high-performance liquid chromatography (HPLC) to more accurately monitor serum creatinine levels in experimental animal models when compared to the Jaffé method. The new HPLC procedure will replace the traditional Jaffé method for rhesus monkey kidney function studies. We developed an isocratic method using a polymeric, hydrophilic, silica-based strong cation-exchange bed with a 5.0 mmol/l lithium acetate matrix, pH 4.9, which… CONTINUE READING